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3rd Annual Workshop

DEPARTMENTS OF PHYSIOLOGY AND BIOENGINEERING

CHRISTIAN MEDICAL COLLEGE
VELLORE
TAMIL NADU 632002

ION TRANSPORT ACROSS MEMBRANES-FROG SKIN

(Voltage-clamp, Current measurements and Fluctuation analysis)

JULY 23, 24   2004

This programme introduces the frog skin, as a model to study membrane transport. Analogies with renal epithelial transport are discussed. The parameters measured are voltages and ionic currents across the membrane with the current-clamp/ Voltage-clamp techniques. Hands on practice on this macroscopic model form the basis for theoretical considerations of ion-channel behaviour, carrier mediated transport kinetics and patch-clamp techniques. The participants also learn principles of designing  physiological salt solutions and techniques to isolate and measure current through a single transport system  across membranes.

  1. Preface
  2. Frog skin electrophysiology
  3. Histology
  4. Voltage Clamp Experiment
  5. Current-Voltage relationship
  6. Preparation of Physiological Solutions
  7. Fluctuation Analysis
  8. Smooth Muscle